Two glycine transporter variants with distinct localization in the CNS and peripheral tissues are encoded by a common gene

Neuron. 1993 May;10(5):851-63. doi: 10.1016/0896-6273(93)90201-2.


We have isolated a cDNA encoding a high affinity, Na+/Cl(-)-dependent glycine transporter, GLYT-2, which is distinct from another glycine transporter, GLYT-1. While the 3' sequences of these two cDNAs are identical, the 5' noncoding regions and the N-termini are completely different. GLYT-1 is found only in the white matter of the CNS, while GLYT-2 is found in the gray matter of the CNS as well as in macrophages and mast cells in peripheral tissues. Our findings suggest that tissue-specific alternative splicing or alternative promoter usage from a single gene results in two mRNA products encoding similar but distinct glycine transporters. The anatomic distribution of GLYT-2 mRNA supports the emerging status of glycine as a supraspinal neurotransmitter and suggests that glycine may function as a chemical messenger outside the CNS.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Amino Acid Transport Systems, Neutral*
  • Animals
  • Base Sequence
  • Brain Chemistry*
  • Carrier Proteins / analysis
  • Carrier Proteins / genetics*
  • Chlorides / pharmacology
  • DNA / analysis
  • Genetic Variation*
  • Glycine Plasma Membrane Transport Proteins
  • Histocytochemistry
  • In Situ Hybridization
  • Leukemia, Basophilic, Acute
  • Mast Cells / chemistry
  • Molecular Sequence Data
  • RNA, Messenger / analysis
  • Rats
  • Sodium / pharmacology
  • Spinal Cord / chemistry*
  • Tissue Distribution
  • Tumor Cells, Cultured


  • Amino Acid Transport Systems, Neutral
  • Carrier Proteins
  • Chlorides
  • Glycine Plasma Membrane Transport Proteins
  • RNA, Messenger
  • Slc6a5 protein, rat
  • Slc6a9 protein, rat
  • DNA
  • Sodium

Associated data

  • GENBANK/L13600