Biosynthesis of corticotropin-releasing hormone in human T-lymphocytes

J Neuroimmunol. 1993 Apr;44(1):7-13. doi: 10.1016/0165-5728(93)90262-w.


Corticotropin-releasing hormone (CRH) is a 41-amino acid neuropeptide which increases the transcription of the proopiomelanocortin (POMC) gene, as well as the biosynthesis and secretion of POMC-derived peptides. Using a specific human CRH radioimmunoassay we have shown that human T-lymphocytes contain immunoreactive CRH. We studied the effects of phytohemagglutinin (PHA) and 12-O-tetradecanoylphorbol-13-acetate (TPA) on the biosynthesis of CRH in human T-lymphocyte cell cultures. A significant increase in CRH mRNA levels was observed in human lymphocytes after 12 h of PHA/TPA treatment, while the levels decreased after 22 h. These findings could imply an immunomodulatory role for CRH that could be due to autocrine and/or paracrine interactions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cells, Cultured
  • Chromatography, High Pressure Liquid
  • Corticotropin-Releasing Hormone / biosynthesis*
  • Humans
  • Nucleic Acid Hybridization
  • Phytohemagglutinins / pharmacology
  • Radioimmunoassay
  • T-Lymphocytes / metabolism*
  • Tetradecanoylphorbol Acetate / pharmacology


  • Phytohemagglutinins
  • Corticotropin-Releasing Hormone
  • Tetradecanoylphorbol Acetate