Effect of beta propiolactone on specific antibody measurements by ELISA

A E Agbarakwe; S A Misbah; H Griffiths; H M Chapel

doi:10.1136/jcp.46.4.368

Effect of beta propiolactone on specific antibody measurements by ELISA

J Clin Pathol. 1993 Apr;46(4):368-9. doi: 10.1136/jcp.46.4.368.

Authors

A E Agbarakwe¹, S A Misbah, H Griffiths, H M Chapel

Affiliation

¹ Department of Immunology, John Radcliffe Hospital, Oxford.

Abstract

Serum samples from 30 HIV seronegative patients were treated with beta propiolactone (BPL) to determine whether BPL interfaces with ELISA for specific antibodies against protein and carbohydrate antigens. BPL had no discernible effect on specific antibody measurements by ELISA. With the measuring need for specific antibody measurements in the management of HIV seropositive patients, it is reassuring that this laboratory safety measure does not impair the reliability of results.

MeSH terms

Antibodies, Bacterial / analysis
Antibodies, Bacterial / drug effects*
Bacterial Capsules
Bacterial Vaccines / immunology
Diphtheria Toxoid / immunology
Enzyme-Linked Immunosorbent Assay*
HIV Infections / immunology*
Haemophilus Vaccines*
Humans
Pneumococcal Vaccines
Polysaccharides, Bacterial / immunology
Propiolactone / pharmacology*
Tetanus Toxoid / immunology

Substances

Antibodies, Bacterial
Bacterial Vaccines
Diphtheria Toxoid
Haemophilus Vaccines
Haemophilus influenzae type b polysaccharide vaccine
Pneumococcal Vaccines
Polysaccharides, Bacterial
Tetanus Toxoid
Propiolactone