Pref-1, a protein containing EGF-like repeats, inhibits adipocyte differentiation

Cell. 1993 May 21;73(4):725-34. doi: 10.1016/0092-8674(93)90252-l.


With the aim of identifying novel regulators of adipocyte differentiation, we have cloned and characterized preadipocyte factor 1 (pref-1), a novel member of the epidermal growth factor (EGF)-like family of proteins. Pref-1 is synthesized as a transmembrane protein with six tandem EGF-like repeats. In preadipocytes, multiple discrete forms of pref-1 protein of 45-60 kd are present, owing in part to N-linked glycosylation. While pref-1 mRNA is abundant in preadipocytes, its expression is completely abolished during differentiation of 3T3-L1 preadipocytes to adipocytes. Moreover, constitutive expression of pref-1 in preadipocytes, which in effect blocks its down-regulation, drastically inhibits adipose differentiation. This indicates that pref-1 functions as a negative regulator of adipocyte differentiation, possibly in a manner analogous to EGF-like proteins that govern cell fate decisions in invertebrates.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adipose Tissue / cytology*
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Calcium-Binding Proteins
  • Cell Differentiation
  • Cell Line
  • Cloning, Molecular
  • Epidermal Growth Factor
  • Intercellular Signaling Peptides and Proteins
  • Membrane Proteins / genetics*
  • Membrane Proteins / physiology
  • Mice
  • Molecular Sequence Data
  • Plasma / physiology
  • RNA, Messenger / biosynthesis
  • Repressor Proteins*
  • Sequence Homology


  • Calcium-Binding Proteins
  • Dlk1 protein, mouse
  • Intercellular Signaling Peptides and Proteins
  • Membrane Proteins
  • RNA, Messenger
  • Repressor Proteins
  • Epidermal Growth Factor

Associated data

  • GENBANK/L12721
  • GENBANK/L14567
  • GENBANK/L14568
  • GENBANK/L14569
  • GENBANK/L33709
  • GENBANK/S60902
  • GENBANK/S60903
  • GENBANK/S60904
  • GENBANK/S60905
  • GENBANK/S60924