FAR1 links the signal transduction pathway to the cell cycle machinery in yeast

Cell. 1993 May 21;73(4):747-60. doi: 10.1016/0092-8674(93)90254-n.


Alpha factor induces arrest of yeast a cells in G1 and transcription of genes involved in mating. Prior work indicates that FUS3, a member of the MAP kinase family, and FAR1, whose molecular activity is unknown, contribute to cell cycle arrest by inhibiting G1 cyclins. Here we show that FAR1 is a substrate for FUS3 and that this phosphorylation regulates association of FAR1 with CDC28-CLN2 kinase. We show also that FAR1 is phosphorylated in vitro by the CDC28-CLN2 complex and in vivo in a CDC28-dependent manner. Mutational analysis of FAR1 reveals a correlation between its ability to associate with CDC28-CLN2 and to arrest the cell cycle. These results suggest that FAR1 protein is the link between the signaling pathway and the cell cycle machinery.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • CDC28 Protein Kinase, S cerevisiae / metabolism
  • Cell Cycle / physiology*
  • Cell Cycle Proteins*
  • Cyclin-Dependent Kinase Inhibitor Proteins
  • DNA Mutational Analysis
  • Fungal Proteins / metabolism*
  • Mating Factor
  • Mitogen-Activated Protein Kinases*
  • Molecular Sequence Data
  • Peptides
  • Phosphorylation
  • Precipitin Tests
  • Protein Binding
  • Repressor Proteins*
  • Saccharomyces cerevisiae / physiology*
  • Saccharomyces cerevisiae Proteins*
  • Signal Transduction / physiology*


  • Cell Cycle Proteins
  • Cyclin-Dependent Kinase Inhibitor Proteins
  • FAR1 protein, S cerevisiae
  • Fungal Proteins
  • Peptides
  • Repressor Proteins
  • Saccharomyces cerevisiae Proteins
  • Mating Factor
  • CDC28 Protein Kinase, S cerevisiae
  • FUS3 protein, S cerevisiae
  • Mitogen-Activated Protein Kinases