Identification of essential components of the S. cerevisiae kinetochore

Cell. 1993 May 21;73(4):761-74. doi: 10.1016/0092-8674(93)90255-o.


We have designed and utilized two in vivo assays of kinetochore integrity in S. cerevisiae. One assay detects relaxation of a transcription block formed at centromeres; the other detects an increase in the mitotic stability of a dicentric test chromosome. ctf13-30 and ctf14-42 were identified as putative kinetochore mutants by both assays. CTF14 is identical to NDC10/CBF2, a recently identified essential gene that encodes a 110 kd kinetochore component. CTF13 is an essential gene that encodes a predicted 478 amino acid protein with no homology to known proteins. ctf13 mutants missegregate chromosomes at permissive temperature and transiently arrest at nonpermissive temperature as large-budded cells with a G2 DNA content and a short spindle. Antibodies recognizing epitope-tagged CTF13 protein decrease the electrophoretic mobility of a CEN DNA-protein complex formed in vitro. Together, the genetic and biochemical data indicate that CTF13 is an essential kinetochore protein.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Centromere / physiology*
  • Cloning, Molecular
  • DNA-Binding Proteins / genetics
  • Fungal Proteins / genetics*
  • Kinetochores
  • Molecular Sequence Data
  • Mutation
  • Nuclear Proteins / genetics*
  • Phenotype
  • Saccharomyces cerevisiae / cytology*
  • Saccharomyces cerevisiae Proteins*


  • CBF2 protein, S cerevisiae
  • CTF13 protein, S cerevisiae
  • DNA-Binding Proteins
  • Fungal Proteins
  • Nuclear Proteins
  • Saccharomyces cerevisiae Proteins

Associated data

  • GENBANK/L10063
  • GENBANK/L14567
  • GENBANK/L14568
  • GENBANK/L14569
  • GENBANK/L33709
  • GENBANK/S60902
  • GENBANK/S60903
  • GENBANK/S60904
  • GENBANK/S60905
  • GENBANK/S60924