The binding of AP-1 clathrin adaptor particles to Golgi membranes requires ADP-ribosylation factor, a small GTP-binding protein

Cell. 1993 Jun 4;73(5):999-1005. doi: 10.1016/0092-8674(93)90277-w.

Abstract

The small GTP-binding protein, ADP-ribosylation factor (ARF), has previously been shown to mediate the binding to Golgi membranes of the coatomer of non-cathrin-coated (COP-coated) vesicles. We now report that ARF is also required for the binding of the AP-1 adaptor protein of clathrin-coated vesicles to Golgi membranes. The binding of coat proteins from both clathrin- and COP-coated vesicles requires an additional Golgi membrane-associated factor. These results suggest that a mechanistic similarity underlies diverse types of vesicle coats.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • ADP-Ribosylation Factors
  • Adaptor Protein Complex alpha Subunits
  • Adaptor Protein Complex gamma Subunits
  • Adaptor Proteins, Vesicular Transport
  • Animals
  • Brain
  • Cattle
  • Clathrin / metabolism
  • Coated Pits, Cell-Membrane / metabolism*
  • GTP-Binding Proteins / physiology*
  • Golgi Apparatus / metabolism*
  • In Vitro Techniques
  • Intracellular Membranes / metabolism
  • Liver
  • Protein Binding
  • Proteins / metabolism*
  • Rats

Substances

  • Adaptor Protein Complex alpha Subunits
  • Adaptor Protein Complex gamma Subunits
  • Adaptor Proteins, Vesicular Transport
  • Clathrin
  • Proteins
  • GTP-Binding Proteins
  • ADP-Ribosylation Factors