Using a simple method of semi-solid agar (0.2%) in U-tubes, the effect of temperature, NaCl, pH and nutrients on the motility of environmental Vibrio cholerae non-01 was elucidated. Under aerobic respiration succinate and pyruvate, which are metabolic intermediates, increased the motility rate, with succinate having the greatest influence. Glucose and benzoic acid reduced the motility rate of most of the strains. Optimum motility was obtained at pH 8.6 and 9.1 in all strains examined. Acidic pH 6.1 and alkaline pH 10.0 reduced motility while the strains showed no growth at pH 4.1. The addition of sodium chloride to the media enhanced motility. The incubation temperature also influenced motility with higher rates recorded at 30 and 37 degrees C than at 25 degrees C.