Release of the outer membrane vesicles from Vibrio cholerae and Vibrio parahaemolyticus

Microbiol Immunol. 1993;37(2):149-52. doi: 10.1111/j.1348-0421.1993.tb03192.x.


We found numerous small vesicles released from the cell by thin sectioning of the plate culture of Vibrio cholerae and V. parahaemolyticus fixed with the freeze-substitution technique. From the broth media of exponentially growing bacteria we could collect the vesicles by the centrifugation but not enough without fixation. The vesicles are encompassed with a membrane structure similar to the outer membrane of these bacteria. The anti-O (Inaba) serum reacted with the surface of the vesicles and the inside of the vesicle are generally filled with an electron-dense mass.

MeSH terms

  • Bacterial Proteins / analysis
  • Cell Membrane / ultrastructure
  • Electrophoresis, Polyacrylamide Gel
  • Microscopy, Electron
  • Organelles / chemistry
  • Organelles / ultrastructure
  • Vibrio cholerae / ultrastructure*
  • Vibrio parahaemolyticus / ultrastructure*


  • Bacterial Proteins