Cloning of the mouse interleukin 2 receptor gamma chain: demonstration of functional differences between the mouse and human receptors

Biochem Biophys Res Commun. 1993 May 28;193(1):356-63. doi: 10.1006/bbrc.1993.1631.


We isolated a cDNA clone for the gamma chain of the mouse interleukin 2 receptor. Introduction of the mouse gamma chain cDNA clone into a mouse fibroblast cell line, L929, expressing the mouse alpha beta heterodimer IL-2 receptor converted pseudo-high affinity of the IL-2 receptor into functional high, resulting in internalization of IL-2 and induction of the c-myc, c-fos and c-jun genes. The mouse beta gamma heterodimer, however, failed to bind IL-2 unlike the human beta gamma heterodimer intermediate-affinity receptor. These results indicate that the mouse functional IL-2 receptor is a complex comprising three distinct subunits, alpha, beta and gamma chains, but the beta gamma heterodimer is not functional and different from the human heterodimer.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Cell Line
  • Cloning, Molecular
  • DNA
  • Humans
  • Interleukin-2 / metabolism
  • Mice
  • Molecular Sequence Data
  • Receptors, Interleukin-2 / chemistry
  • Receptors, Interleukin-2 / genetics*
  • Receptors, Interleukin-2 / metabolism
  • Sequence Homology, Amino Acid
  • Species Specificity


  • Interleukin-2
  • Receptors, Interleukin-2
  • DNA

Associated data

  • GENBANK/D13565
  • GENBANK/D14599
  • GENBANK/D14600
  • GENBANK/D14601
  • GENBANK/D14602
  • GENBANK/D14603
  • GENBANK/L16579
  • GENBANK/S61383
  • GENBANK/S64636
  • GENBANK/S64637