Increased expression of endothelin-1 gene in blood vessels of deoxycorticosterone acetate-salt hypertensive rats

Hypertension. 1993 Jun;21(6 Pt 2):916-20. doi: 10.1161/01.hyp.21.6.916.

Abstract

We have recently shown that the content of immunoreactive endothelin-1 is increased in acid extracts from blood vessels of deoxycorticosterone acetate (DOCA)-salt hypertensive rats compared with uninephrectomized control rats. We have also found by immunohistochemistry a significant increase in immunoreactive endothelin-1 in endothelial cells of aorta and mesenteric arteries of DOCA-salt hypertensive rats. In the present study, we investigated preproendothelin-1 gene expression in blood vessels of DOCA-salt hypertensive rats and uninephrectomized control rats. Northern blot analysis using a specific 32P-labeled complementary RNA probe for rat preproendothelin-1 of 319 base pairs revealed a fourfold to fivefold increase in abundance of preproendothelin-1 messenger RNA transcripts in both aorta and mesenteric arteries from DOCA-salt hypertensive rats. Thus, increased immunoreactive endothelin-1 content in blood vessels of DOCA-salt hypertensive rats is secondary to increased preproendothelin-1 gene expression. Exaggerated expression of the preproendothelin-1 gene in mineralocorticoid hypertension may contribute to the maintenance of elevated blood pressure.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Blood Pressure
  • Blood Vessels / physiopathology
  • Blotting, Northern
  • Desoxycorticosterone
  • Endothelin-1
  • Endothelins / genetics*
  • Gene Expression*
  • Hypertension / chemically induced*
  • Hypertension / genetics*
  • Hypertension / physiopathology
  • Male
  • Molecular Probes / genetics
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Protein Precursors / genetics
  • RNA, Messenger / metabolism
  • Rats
  • Rats, Sprague-Dawley
  • Sodium Chloride

Substances

  • Endothelin-1
  • Endothelins
  • Molecular Probes
  • Protein Precursors
  • RNA, Messenger
  • Desoxycorticosterone
  • Sodium Chloride