Evaluation of an ELISA for detection of ovine progressive pneumonia antibodies using a recombinant transmembrane envelope protein

J Vet Diagn Invest. 1993 Apr;5(2):189-93. doi: 10.1177/104063879300500208.

Abstract

An enzyme-linked immunosorbent assay (ELISA) was developed using a recombinant protein corresponding to the N'-terminal hydrophilic region of transmembrane glycoprotein (TM) of ovine lentivirus. This assay reproducibly detected antibodies in sera from 207 of 212 ovine progressive pneumonia (OPP) virus-infected sheep, and the recombinant TM ELISA accurately identified 26% (35 vs. 9) more seropositive samples than did the agar gel immunodiffusion test when applied to 100 sera from an infected flock. This assay also yielded no false-positive results in 14 true negative sera. Results of these experiments were further confirmed by the recombinant TM and recombinant p25 Western blot assay. A single recombinant TM antigen, as the coating antigen in ELISA, can be used successfully for the detection of OPP virus-infected animals and can improve the sensitivity and specificity for OPP diagnosis.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Antibodies, Viral / blood*
  • Enzyme-Linked Immunosorbent Assay / methods
  • Enzyme-Linked Immunosorbent Assay / veterinary*
  • Evaluation Studies as Topic
  • Glutathione Transferase
  • Immunodiffusion / veterinary
  • Pneumonia, Progressive Interstitial, of Sheep / diagnosis*
  • Pneumonia, Viral / microbiology
  • Pneumonia, Viral / veterinary*
  • Recombinant Fusion Proteins
  • Sheep

Substances

  • Antibodies, Viral
  • Recombinant Fusion Proteins
  • Glutathione Transferase