Expression of mRNA for the GATA-binding proteins in human eosinophils and basophils: potential role in gene transcription

Blood. 1993 Jun 15;81(12):3234-41.

Abstract

The expression of the hematopoietic transcription factors GATA-1, GATA-2, and GATA-3 was studied in eosinophils and basophils. Eosinophils express mRNA for GATA-1, GATA-2, and GATA-3. Basophils express GATA-2 and GATA-3. Treatment of HL-60 eosinophilic sublines with either interleukin-5 or butyric acid increased the expression of GATA-1 mRNA concomitant with the expression of eosinophil-specific genes, whereas levels of GATA-2 mRNA remained relatively constant. The presence of mRNA for these proteins in eosinophils and basophils suggests that gene transcription in these lineages may be regulated by GATA-binding proteins.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • Basophils / metabolism*
  • Blotting, Northern
  • Butyrates / pharmacology
  • Butyric Acid
  • Cell Line
  • DNA-Binding Proteins / genetics*
  • Eosinophils / metabolism*
  • Erythroid-Specific DNA-Binding Factors
  • GATA1 Transcription Factor
  • GATA2 Transcription Factor
  • GATA3 Transcription Factor
  • Gene Expression* / drug effects
  • HeLa Cells
  • Humans
  • Interleukin-5 / pharmacology
  • Molecular Sequence Data
  • RNA, Messenger / biosynthesis*
  • Trans-Activators / genetics*
  • Transcription Factors / genetics*
  • Transcription, Genetic

Substances

  • Butyrates
  • DNA-Binding Proteins
  • Erythroid-Specific DNA-Binding Factors
  • GATA1 Transcription Factor
  • GATA1 protein, human
  • GATA2 Transcription Factor
  • GATA2 protein, human
  • GATA3 Transcription Factor
  • GATA3 protein, human
  • Interleukin-5
  • RNA, Messenger
  • Trans-Activators
  • Transcription Factors
  • Butyric Acid