Abstract
The expression of the hematopoietic transcription factors GATA-1, GATA-2, and GATA-3 was studied in eosinophils and basophils. Eosinophils express mRNA for GATA-1, GATA-2, and GATA-3. Basophils express GATA-2 and GATA-3. Treatment of HL-60 eosinophilic sublines with either interleukin-5 or butyric acid increased the expression of GATA-1 mRNA concomitant with the expression of eosinophil-specific genes, whereas levels of GATA-2 mRNA remained relatively constant. The presence of mRNA for these proteins in eosinophils and basophils suggests that gene transcription in these lineages may be regulated by GATA-binding proteins.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Base Sequence
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Basophils / metabolism*
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Blotting, Northern
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Butyrates / pharmacology
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Butyric Acid
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Cell Line
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DNA-Binding Proteins / genetics*
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Eosinophils / metabolism*
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Erythroid-Specific DNA-Binding Factors
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GATA1 Transcription Factor
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GATA2 Transcription Factor
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GATA3 Transcription Factor
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Gene Expression* / drug effects
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HeLa Cells
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Humans
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Interleukin-5 / pharmacology
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Molecular Sequence Data
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RNA, Messenger / biosynthesis*
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Trans-Activators / genetics*
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Transcription Factors / genetics*
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Transcription, Genetic
Substances
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Butyrates
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DNA-Binding Proteins
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Erythroid-Specific DNA-Binding Factors
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GATA1 Transcription Factor
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GATA1 protein, human
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GATA2 Transcription Factor
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GATA2 protein, human
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GATA3 Transcription Factor
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GATA3 protein, human
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Interleukin-5
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RNA, Messenger
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Trans-Activators
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Transcription Factors
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Butyric Acid