An alternative dystrophin transcript specific to peripheral nerve

Nat Genet. 1993 May;4(1):77-81. doi: 10.1038/ng0593-77.

Abstract

Transcription of the 2.5 megabase dystrophin gene gives rise to multiple isoforms. We describe a 5.2 kilobase transcript, expressed specifically in peripheral nerve, that initiates at a previously unrecognized exon located approximately 850 basepairs upstream of dystrophin exon 56. The likely product of this transcript (Dp116) is detected by C-terminal dystrophin antibodies exclusively in peripheral nerve and cultured Schwann cells. Dp116 is located along the Schwann cell membrane but is not present in the compact myelin lamellae or in axons. Dp116 lacks actin-binding and spectrin-like rod domains, arguing that it functions differently in the Schwann cell than does the major dystrophin transcript in muscle.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Dystrophin / genetics*
  • Exons*
  • Gene Expression Regulation
  • Gene Library
  • Humans
  • Molecular Sequence Data
  • Nerve Tissue Proteins / genetics*
  • Organ Specificity
  • Peripheral Nerves / chemistry*
  • RNA Splicing
  • RNA, Messenger / analysis
  • RNA, Messenger / genetics*
  • Rats
  • Sciatic Nerve / chemistry
  • Transcription, Genetic*

Substances

  • Dystrophin
  • Nerve Tissue Proteins
  • RNA, Messenger