Rhizopodin, a new compound from Myxococcus stipitatus (myxobacteria) causes formation of rhizopodia-like structures in animal cell cultures. Production, isolation, physico-chemical and biological properties
- PMID: 8514628
- DOI: 10.7164/antibiotics.46.741
Rhizopodin, a new compound from Myxococcus stipitatus (myxobacteria) causes formation of rhizopodia-like structures in animal cell cultures. Production, isolation, physico-chemical and biological properties
Abstract
A new cytostatic compound, rhizopodin, was isolated from the culture broth of the myxobacterium, Myxococcus stipitatus. The compound inhibited growth of various animal cell cultures without killing the cells. The ID50, measured by an MTT assay, was 12 approximately 30 ng/ml, depending on the cell line. Especially cells growing fibroblast-like showed typical morphological changes. They became larger and within hour formed long branching and reticular runners. These morphological changes were irreversible. Rhizopodin suppresses bleb formation in K-562 cells, and therefore could act by interacting with protein phosphorylation.
Similar articles
-
Configurational assignment of rhizopodin, an actin-binding macrolide from the myxobacterium Myxococcus stipitatus.Chem Commun (Camb). 2008 Nov 7;(41):5173-5. doi: 10.1039/b810405k. Epub 2008 Sep 9. Chem Commun (Camb). 2008. PMID: 18956059
-
Two new ring-contracted congeners of rhizopodin illustrate significance of the ring moiety of macrolide toxins on the actin disassembly-mediated cytotoxicity.Chem Pharm Bull (Tokyo). 2014;62(3):294-300. doi: 10.1248/cpb.c13-00856. Chem Pharm Bull (Tokyo). 2014. PMID: 24583785
-
Formal synthesis of actin binding macrolide rhizopodin.Org Lett. 2014 Apr 18;16(8):2284-7. doi: 10.1021/ol5008179. Epub 2014 Apr 10. Org Lett. 2014. PMID: 24742162
-
Effects of rhizopodin and latrunculin B on the morphology and on the actin cytoskeleton of mammalian cells.Cell Tissue Res. 1999 Jan;295(1):121-9. doi: 10.1007/s004410051218. Cell Tissue Res. 1999. PMID: 9931358
-
Calyculin: Nature's way of making the sponge-derived cytotoxin.Nat Prod Rep. 2016 Jun 2;33(6):751-60. doi: 10.1039/c5np00123d. Nat Prod Rep. 2016. PMID: 26923942 Review.
Cited by
-
Identification of myxobacteria-derived HIV inhibitors by a high-throughput two-step infectivity assay.Microb Cell Fact. 2013 Sep 24;12:85. doi: 10.1186/1475-2859-12-85. Microb Cell Fact. 2013. PMID: 24063434 Free PMC article.
-
Design, Synthesis, and Biological Evaluation of Novel 1,3-Oxazole Sulfonamides as Tubulin Polymerization Inhibitors.ACS Med Chem Lett. 2021 May 25;12(6):1030-1037. doi: 10.1021/acsmedchemlett.1c00219. eCollection 2021 Jun 10. ACS Med Chem Lett. 2021. PMID: 34141089 Free PMC article.
-
Isolation, biology and chemistry of the disorazoles: new anti-cancer macrodiolides.Nat Prod Rep. 2009 May;26(5):585-601. doi: 10.1039/b813799b. Epub 2009 Mar 11. Nat Prod Rep. 2009. PMID: 19387496 Free PMC article. Review.
-
Challenges and discoveries in the total synthesis of complex polyketide natural products.J Antibiot (Tokyo). 2018 Feb;71(2):215-233. doi: 10.1038/ja.2017.111. Epub 2017 Oct 25. J Antibiot (Tokyo). 2018. PMID: 29066790 Review.
-
An Effective Bifunctional Aldehyde Linchpin for Type II Anion Relay Chemistry: Development and Application to the Synthesis of a C16-C29 Fragment of Rhizopodin.Org Lett. 2015 Dec 18;17(24):6242-5. doi: 10.1021/acs.orglett.5b03235. Epub 2015 Dec 7. Org Lett. 2015. PMID: 26641664 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
