Increased phagocytosis and generation of reactive oxygen products by neutrophils and monocytes of men with stage 1 human immunodeficiency virus infection

J Infect Dis. 1993 Jul;168(1):75-83. doi: 10.1093/infdis/168.1.75.


Flow cytometry was used to study phagocytic function and release of reactive oxygen products following phagocytosis by neutrophils (PMNL) and monocytes of heparinized whole blood from stage 1 human immunodeficiency virus type 1 (HIV-1)-infected men. Phagocytic capacity was assessed by measuring uptake of Texas red-labeled bacteria. Reactive oxygen generation after phagocytosis was estimated by the quantity of dichlorofluorescein diacetate converted to dichlorofluorescein intracellularly. Compared with results in samples from age- and sex-matched controls, PMNL and monocytes from HIV-1-infected patients exhibited a significantly increased capacity to phagocytose Staphylococcus aureus and Escherichia coli and generate reactive oxygen products. These results are consistent with the hypothesis that stimuli associated with early HIV-1 infection enhance the nonspecific response of phagocytic cells to potential bacterial pathogens.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Flow Cytometry
  • HIV Infections / metabolism*
  • HIV-1*
  • Humans
  • Male
  • Monocytes / metabolism*
  • Neutrophils / metabolism*
  • Phagocytosis*
  • Reactive Oxygen Species / metabolism*


  • Reactive Oxygen Species