Chimerism and donor-specific nonreactivity 27 to 29 years after kidney allotransplantation

Transplantation. 1993 Jun;55(6):1272-7. doi: 10.1097/00007890-199306000-00012.


Chimerism was demonstrated with immunocytochemical and/or polymerase chain reaction techniques in kidney allografts and in the native skin, lymph nodes, or blood of 5 of 5 patients who received continuously functioning renal transplants from 1 or 2 haplotype HLA mismatched consanguineous donors (4 parents, 1 aunt) 27-29 years ago. In the 4 cases where the kidney donor still was alive to provide stimulator lymphocytes for testing, these provoked no (n = 2) or modest (n = 2) MLR in contrast to vigorous MLR to third party lymphocytes. In all 4 cases, the donor cells failed to generate in vitro cytotoxic effector cells (cell-mediated lymphocytotoxicity). These findings are in accord with the hypothesis that cell migration, repopulation, and chimerism are seminal events that define graft acceptance and ultimately can lead to acquired donor-specific nonresponsiveness (tolerance).

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adult
  • Chimera
  • Female
  • Graft Survival*
  • HLA-DR Antigens / analysis
  • Humans
  • Immune Tolerance
  • Kidney Transplantation / immunology*
  • Kidney Transplantation / pathology*
  • Lymphocyte Culture Test, Mixed
  • Male
  • Polymerase Chain Reaction
  • Time Factors


  • HLA-DR Antigens