The clinical significance of allospecific antibodies against endothelial cells detected with an antibody-dependent cellular cytotoxicity assay for vascular rejection and graft loss after renal transplantation

Transplantation. 1993 Jun;55(6):1287-93. doi: 10.1097/00007890-199306000-00015.


Serum samples of 64 consecutive patients who underwent renal transplantation in our institution were examined for the presence of antibody-dependent cellular cytotoxicity (ADCC) activity against endothelial cells (EC). From each patient serum samples were obtained immediately before transplantation and 1 week, 1 month and 1 year thereafter. The results were evaluated in the context of tests to measure donor-specific humoral immunity against lymphocytes and monocytes, and related to parameters of presensitization, graft survival, and histology. Sera from 10 patients were positive for ADCC on a panel of HLA-typed endothelial cells. In 8 patients sera were already positive before transplantation and remained positive thereafter. In 4 patients a positive crossmatch with donor T and B cells and monocytes could be observed after transplantation. In only one patient were these crossmatches positive before transplantation. A significant correlation was found between ADCC positivity and vascular rejection (P = 0.015); in addition graft survival was significantly better in the ADCC negative group vs. the positive group (P = 0.0004). These data demonstrate the significance of allospecific anti EC antibodies for the occurrence of vascular rejection and graft loss after renal transplantation.

Publication types

  • Clinical Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Antibody-Dependent Cell Cytotoxicity
  • Endothelium, Vascular / immunology*
  • Female
  • Graft Rejection*
  • Graft Survival*
  • Histocompatibility Antigens Class I / analysis
  • Histocompatibility Testing
  • Humans
  • Isoantibodies / analysis
  • Isoantibodies / immunology
  • Kidney Transplantation / immunology*
  • Male
  • Middle Aged


  • Histocompatibility Antigens Class I
  • Isoantibodies