Expression of nm23-H1 and nm23-H2 protein in endometrial carcinoma

Br J Cancer. 1995 Dec;72(6):1469-73. doi: 10.1038/bjc.1995.531.

Abstract

nm23 gene expression has been shown to be inversely correlated with tumour metastatic potential in some cancers but not in others. Examination was made of the expression of nm23-H1 and nm23-H2 gene products by immunohistochemistry and immunoblotting in 28 endometrial carcinomas. Immunohistochemistry indicated the cytoplasm of cancer cells to be positive, and myometrium and endometrial stromal cells negative, for nm23-H1 and -H2 protein. The staining intensity for these proteins was significantly stronger in well-differentiated adenocarcinomas (G1) than in those moderately differentiated (G2) (P < 0.05). nm23-H1 and -H2 proteins were shown by immunoblotting to be present at significantly higher levels in G1 than in G2 tumours (P < 0.05). Two of eight cases expressed high nm23-H1 and -H2 protein in poorly differentiated adenocarcinomas (G3). In G3 tumours, nm23 expression may be diverse. In this study, the expression of nm23-H1 and -H2 was not correlated with stage, metastasis, tumour size, myometrial invasion, oestrogen receptor, progesterone receptor or menopause. It follows from the findings presented above that the high expression of nm23-H1 and -H2 is positively correlated with histological differentiation.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Animals
  • Cell Differentiation / physiology
  • Endometrial Neoplasms / chemistry*
  • Endometrial Neoplasms / pathology
  • Endometrial Neoplasms / ultrastructure
  • Female
  • Humans
  • Immunoblotting
  • Immunohistochemistry
  • Menopause
  • Mice
  • Middle Aged
  • Monomeric GTP-Binding Proteins*
  • NM23 Nucleoside Diphosphate Kinases
  • Neoplasm Metastasis
  • Nucleoside-Diphosphate Kinase / biosynthesis
  • Receptors, Estrogen / analysis
  • Receptors, Progesterone / analysis
  • Transcription Factors / biosynthesis*

Substances

  • NM23 Nucleoside Diphosphate Kinases
  • Receptors, Estrogen
  • Receptors, Progesterone
  • Transcription Factors
  • NME1 protein, human
  • Nme1 protein, mouse
  • Nucleoside-Diphosphate Kinase
  • Monomeric GTP-Binding Proteins