The interaction of 1-anilino-8-naphthalenesulfonate with lipids: a nuclear magnetic resonance study

Chem Phys Lipids. 1977 Mar;18(2):181-98. doi: 10.1016/0009-3084(77)90005-6.


The proton magnetic resonance (PMR) and phosphorus magnetic resonance (PhMR) spectra of egg phosphatidylcholine in the presence of 1-anilino-8-nahthalenesulfonate (ANS) have been studied. At low ratios of ANS to phospholipid, the spectra indicate that ANS molecules are in the lipid interface region where they interact with the head-group protons. ANS also penetrates into the hydrocarbon region to some extent. As the ANS/phospholipid ratio approaches one, a significant splitting of the head-group signal occurs. This splitting is associated with head-group signals from inner and outer molecules of the phospholipid vesicles. As the ANS/phospholipid ratio is further increased, a gel phase often occurs. The spectra for this gel phase suggest a highly mobile head-group. Further ANS addition results in a PMR spectrum suggestive of ANS-phospholipid micelle formation. The results for a phospholipid-cholesterol complex and for the total lipid extract from a cell membrane show that the ANS effect is more complicated in these cases.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Anilino Naphthalenesulfonates*
  • Animals
  • Binding Sites
  • Cell Membrane / analysis
  • Magnetic Resonance Spectroscopy
  • Membrane Lipids / analysis
  • Molecular Conformation
  • Phosphatidylcholines*
  • Thyroid Gland / analysis


  • Anilino Naphthalenesulfonates
  • Membrane Lipids
  • Phosphatidylcholines