Precise large deletions by the PCR-based overlap extension method

Mol Biotechnol. 1995 Aug;4(1):13-5. doi: 10.1007/BF02907467.


The authors describe an efficient method for generating large deletions (> 200 nts) of precise length using the PCR-based method of gene splicing by overlap extension (1). This method is technically simple and less time consuming than conventional loop-out mutagenesis techniques requiring preparation of a single-stranded DNA template.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • Cloning, Molecular
  • Coronavirus / genetics
  • DNA Primers
  • DNA, Recombinant
  • Molecular Sequence Data
  • Mutagenesis, Site-Directed*
  • Polymerase Chain Reaction / methods*
  • RNA / chemistry
  • RNA, Viral
  • Sequence Deletion*


  • DNA Primers
  • DNA, Recombinant
  • RNA, Viral
  • RNA, recombinant
  • RNA