Identification of Double Holliday Junctions as Intermediates in Meiotic Recombination

Cell. 1995 Dec 1;83(5):783-91. doi: 10.1016/0092-8674(95)90191-4.

Abstract

During meiosis, branched DNA molecules containing information from both parental chromosomes occur in vivo at loci where meiosis-specific double-stranded breaks occur. We demonstrate here that these joint molecules are recombination intermediates: they contain single strands that have undergone exchange of information. Moreover, these joint molecules are resolved into both parental and recombinant duplexes when treated in vitro with Holliday junction-resolving endonucleases RuvC or T4 endo VII. Taken together with previous observations, these results strongly suggest that joint molecules are double Holliday junctions.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alcohol Oxidoreductases
  • Aminohydrolases
  • Bacterial Proteins
  • Cross-Linking Reagents
  • Crossing Over, Genetic / genetics
  • DNA, Fungal / chemistry
  • DNA, Fungal / genetics
  • DNA, Single-Stranded / chemistry
  • DNA, Single-Stranded / genetics*
  • Endodeoxyribonucleases
  • Escherichia coli Proteins*
  • Ficusin
  • Fungal Proteins / genetics
  • Meiosis / genetics*
  • Models, Genetic
  • Nucleic Acid Conformation
  • Nucleic Acid Heteroduplexes / genetics
  • Pyrophosphatases
  • Recombination, Genetic / genetics*
  • Saccharomyces cerevisiae Proteins*
  • Transcription Factors / genetics

Substances

  • Bacterial Proteins
  • Cross-Linking Reagents
  • DNA, Fungal
  • DNA, Single-Stranded
  • Escherichia coli Proteins
  • Fungal Proteins
  • Nucleic Acid Heteroduplexes
  • Saccharomyces cerevisiae Proteins
  • Transcription Factors
  • ruvC protein, E coli
  • Alcohol Oxidoreductases
  • HIS4 protein, S cerevisiae
  • Endodeoxyribonucleases
  • endodeoxyribonuclease VII
  • Aminohydrolases
  • Pyrophosphatases
  • Ficusin