Regional assignment of the human 4-hydroxyphenylpyruvate dioxygenase gene (HPD) to 12q24-->qter by fluorescence in situ hybridization

Cytogenet Cell Genet. 1995;71(4):374-6. doi: 10.1159/000134142.

Abstract

Using a panel of human-rodent somatic cell hybrids, we have previously mapped the gene (HPD, previously called PPD) encoding 4-hydroxyphenylpyruvate dioxygenase to the distal half of the long arm of human chromosome 12, region q14-->qter. To obtain a genomic probe useful for fluorescence in situ hybridization (FISH) analysis we screened a human leukocyte genomic library and isolated a 13.4-kb phage clone, which by restriction fragment and sequence analyses was shown to contain exons 1-10 of HPD and approximately 2-kb upstream sequences. We now report the subregional localization of HPD to 12q24-->qter based on two color FISH analysis employing this clone.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 4-Hydroxyphenylpyruvate Dioxygenase / genetics*
  • Animals
  • Base Sequence
  • Blotting, Southern
  • Chromosome Mapping
  • Chromosomes, Human, Pair 12 / genetics*
  • Chromosomes, Human, Pair 12 / ultrastructure
  • DNA, Complementary
  • Exons / genetics
  • Humans
  • In Situ Hybridization, Fluorescence
  • Mice
  • Molecular Sequence Data

Substances

  • DNA, Complementary
  • 4-Hydroxyphenylpyruvate Dioxygenase

Associated data

  • GENBANK/U29895