A subset of HLA-DR9 molecules is detected by a polymorphic monoclonal antibody on lymphoblastoid cell lines but not on peripheral blood lymphocytes

Hum Immunol. 1995 Sep;44(1):19-27. doi: 10.1016/0198-8859(95)00045-6.


Some mAbs recognizing polymorphic epitopes of HLA-DR molecules exhibit striking differences of reactivity with the same HLA-DR molecules expressed by different cell types. In this study, we investigated the basis for the differential reactivity of the polymorphic anti-DR mAb OHA TM901 with HLA-DR9 molecules expressed by human PBLs or LCLs. By immunoprecipitation experiments we showed that OHA TM901 recognizes a subset of HLA-DR9 molecules from LCLs. This subset corresponds to HLA-DR9 molecules containing immature-type oligosaccharides. The absence of OHA TM901 reactivity with HLA-DR9 PBLs, as revealed by cytofluorometry analysis, suggests that this subset is either not expressed or expressed at a very low level on PBLs. These results indicate that overexpression of HLA-DR molecules in immortalized LCLs could lead to cell-surface expression of underglycosylated forms which are generally not found on the cell surface of PBLs.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Monoclonal / immunology*
  • B-Lymphocytes / immunology
  • Blood Cells
  • Cell Line, Transformed
  • Epitopes / immunology
  • HLA-DR Antigens / analysis*
  • HLA-DR Antigens / chemistry
  • HLA-DR Antigens / classification
  • HLA-DR Antigens / immunology
  • HLA-DR Serological Subtypes
  • Humans
  • Lymphocyte Subsets / immunology*
  • Neuraminidase / pharmacology
  • Oligosaccharides / analysis
  • Sialic Acids / analysis


  • Antibodies, Monoclonal
  • Epitopes
  • HLA-DR Antigens
  • HLA-DR Serological Subtypes
  • HLA-DR9 antigen
  • Oligosaccharides
  • Sialic Acids
  • Neuraminidase