Simian immunodeficiency virus-specific cytotoxic T-lymphocyte induction through DNA vaccination of rhesus monkeys

J Virol. 1996 Jan;70(1):678-81. doi: 10.1128/JVI.70.1.678-681.1996.


In view of the growing evidence that virus-specific cytotoxic T lymphocytes (CTL) play an important role in containing the early spread of human immunodeficiency virus type 1 (HIV-1) in infected individuals, novel vaccine strategies capable of eliciting HIV-1-specific CTL are being pursued in attempts to create an effective AIDS vaccine. We have used the simian immunodeficiency virus of macaques (SIVmac)/rhesus monkey model to explore the induction of AIDS virus-specific CTL responses by DNA vaccination. We found that the inoculation of rhesus monkeys with plasmid DNA encoding SIVmac Env and Gag elicited a persisting SIVmac-specific memory CTL response. These CTL were CD8+ and major histocompatibility complex class I restricted. These studies provide evidence for the potential utility of DNA inoculation as an approach to an HIV-1 vaccine.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • DNA, Viral / administration & dosage
  • DNA, Viral / immunology*
  • Gene Products, env / genetics
  • Gene Products, env / immunology
  • Histocompatibility Antigens Class I / immunology
  • Macaca mulatta
  • Plasmids
  • Retroviridae Proteins / genetics
  • Retroviridae Proteins / immunology
  • Simian Immunodeficiency Virus / genetics
  • Simian Immunodeficiency Virus / immunology*
  • T-Lymphocytes, Cytotoxic / immunology*
  • Tumor Cells, Cultured
  • Vaccines, Synthetic / administration & dosage
  • Vaccines, Synthetic / immunology
  • Viral Envelope Proteins / genetics
  • Viral Envelope Proteins / immunology
  • Viral Vaccines / administration & dosage
  • Viral Vaccines / immunology*


  • DNA, Viral
  • Gene Products, env
  • Histocompatibility Antigens Class I
  • Retroviridae Proteins
  • SIV envelope protein gp110
  • Vaccines, Synthetic
  • Viral Envelope Proteins
  • Viral Vaccines
  • simian immunodeficiency virus gp130