Ras-Raf interaction: two-hybrid analysis

Methods Enzymol. 1995;255:331-42. doi: 10.1016/s0076-6879(95)55036-4.

Abstract

The identification of proteins mediating Ras effects, such as the serine/threonine kinases of the Raf family, has advanced our understanding of how signals are transmitted from the extracellular milieu to the nucleus. The modified two-hybrid system has proved to be a powerful tool for identifying specific protein interactions, such as those between Ras and Raf. We hope that the insight gained from the Ras screen, as well as insights from other two hybrid screens, will prove valuable in the application of this system to other enigmatic questions in biology.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular
  • DNA Primers / genetics
  • Gene Library
  • Genetic Techniques
  • Mice
  • Molecular Sequence Data
  • Plasmids / genetics
  • Protein-Serine-Threonine Kinases / analysis
  • Protein-Serine-Threonine Kinases / genetics
  • Protein-Serine-Threonine Kinases / metabolism*
  • Proto-Oncogene Proteins / analysis
  • Proto-Oncogene Proteins / genetics
  • Proto-Oncogene Proteins / metabolism*
  • Proto-Oncogene Proteins c-raf
  • Recombinant Fusion Proteins / analysis
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Saccharomyces cerevisiae / genetics
  • Transformation, Genetic
  • ras Proteins / analysis
  • ras Proteins / genetics
  • ras Proteins / metabolism*

Substances

  • DNA Primers
  • Proto-Oncogene Proteins
  • Recombinant Fusion Proteins
  • Protein-Serine-Threonine Kinases
  • Proto-Oncogene Proteins c-raf
  • ras Proteins