Analysis of intrinsic and CDC25-stimulated guanine nucleotide exchange of p21ras-nucleotide complexes by fluorescence measurements

Methods Enzymol. 1995:255:95-109. doi: 10.1016/s0076-6879(95)55012-7.
No abstract available

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Cycle Proteins / genetics
  • Cell Cycle Proteins / isolation & purification
  • Cell Cycle Proteins / metabolism*
  • Chromatography, High Pressure Liquid
  • Escherichia coli / genetics
  • Fluorescent Dyes / chemical synthesis
  • Fluorescent Dyes / chemistry
  • Fluorescent Dyes / metabolism
  • Guanine Nucleotide Exchange Factors
  • Guanine Nucleotides / chemical synthesis
  • Guanine Nucleotides / chemistry
  • Guanine Nucleotides / metabolism*
  • In Vitro Techniques
  • Kinetics
  • Mice
  • Phosphoprotein Phosphatases / genetics
  • Phosphoprotein Phosphatases / isolation & purification
  • Phosphoprotein Phosphatases / metabolism*
  • Proteins / genetics
  • Proteins / isolation & purification
  • Proteins / metabolism
  • Proto-Oncogene Proteins p21(ras) / metabolism*
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / isolation & purification
  • Recombinant Fusion Proteins / metabolism
  • Spectrometry, Fluorescence
  • ortho-Aminobenzoates / chemical synthesis
  • ortho-Aminobenzoates / chemistry
  • ortho-Aminobenzoates / metabolism
  • ras Guanine Nucleotide Exchange Factors
  • ras-GRF1

Substances

  • Cell Cycle Proteins
  • Fluorescent Dyes
  • Guanine Nucleotide Exchange Factors
  • Guanine Nucleotides
  • Proteins
  • Recombinant Fusion Proteins
  • ortho-Aminobenzoates
  • ras Guanine Nucleotide Exchange Factors
  • ras-GRF1
  • Phosphoprotein Phosphatases
  • Proto-Oncogene Proteins p21(ras)