Nucleation of Microtubule Assembly by a Gamma-Tubulin-Containing Ring Complex

Nature. 1995 Dec 7;378(6557):578-83. doi: 10.1038/378578a0.

Abstract

The highly conserved protein gamma-tubulin is required for microtubule nucleation in vivo. When viewed in the electron microscope, a highly purified gamma-tubulin complex from Xenopus consisting of at least seven different proteins is seen to have an open ring structure. This complex acts as an active microtubule-nucleating unit which can cap the minus ends of microtubules in vitro.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Chromatography, Affinity
  • Microscopy, Electron
  • Microtubules / metabolism*
  • Microtubules / ultrastructure
  • Molecular Sequence Data
  • Oocytes / chemistry
  • Protein Conformation
  • Protein Structure, Secondary
  • Tubulin / chemistry*
  • Tubulin / isolation & purification
  • Tubulin / metabolism
  • Tubulin / ultrastructure
  • Xenopus

Substances

  • Tubulin