Alternative translation initiation site usage results in two functionally distinct forms of the GATA-1 transcription factor

Proc Natl Acad Sci U S A. 1995 Dec 5;92(25):11598-602. doi: 10.1073/pnas.92.25.11598.

Abstract

GATA-1 is a zinc-finger transcription factor that plays a critical role in the normal development of hematopoietic cell lineages. In human and murine erythroid cells a previously undescribed 40-kDa protein is detected with GATA-1-specific antibodies. We show that the 40-kDa GATA-1 (GATA-1s) is produced by the use of an internal AUG initiation codon in the GATA-1 transcript. The GATA-1 proteins share identical binding activity and form heterodimers in erythroleukemic cells but differ in their transactivation potential and in their expression in developing mouse embryos.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Cells, Cultured
  • DNA-Binding Proteins / genetics*
  • DNA-Binding Proteins / metabolism
  • Embryo, Mammalian / metabolism
  • Erythroid-Specific DNA-Binding Factors
  • GATA1 Transcription Factor
  • Genetic Variation*
  • Humans
  • Mice
  • Molecular Sequence Data
  • Molecular Weight
  • Peptide Chain Initiation, Translational / genetics*
  • Protein Binding
  • Protein Conformation
  • Tissue Distribution
  • Transcription Factors / genetics*
  • Transcription Factors / metabolism
  • Transcriptional Activation

Substances

  • DNA-Binding Proteins
  • Erythroid-Specific DNA-Binding Factors
  • GATA1 Transcription Factor
  • GATA1 protein, human
  • Gata1 protein, mouse
  • Transcription Factors