Acoustic energy determines haemoglobin release from erythrocytes by extracorporeal shock waves in vitro

Ultrasound Med Biol. 1995;21(5):707-10. doi: 10.1016/0301-5629(94)00155-7.


Haemoglobin release from erythrocytes by extracorporeal shock waves from an electrohydraulic lithotripter was quantified and correlated with the acoustic energy administered to the cell container. Cells were exposed in 2-, 5.9-, and 10.5-mL vials to 100 shock waves delivered at a low, medium and high lithotripter output setting, both with and without covering of the central ellipsoidal axis by a metal cage. Using the identical set-up, previous experiments had shown that the fragmentation efficiency was linearly correlated with the delivered acoustic energy. As a result, shock waves generated from 0.83 microgram mJ-1 (in 2-mL vials) to 1.53 micrograms mJ-1 (in 10.5-mL vials) haemoglobin. At all vial types, the amount of haemoglobin correlated linearly with the delivered acoustic energy (r = 0.96 in 2-mL, r = 0.97 in 5.9-mL and r = 0.98 in 10.5-mL vials). It was independent of the presence of the cage.

MeSH terms

  • Acoustics
  • Cell Membrane Permeability
  • Erythrocytes / metabolism*
  • Hemoglobins / metabolism*
  • Hemolysis*
  • Humans
  • Lithotripsy / adverse effects*


  • Hemoglobins