Dendritic cells differentiated from human monocytes through a combination of IL-4, GM-CSF and IFN-gamma exhibit phenotype and function of blood dendritic cells

Adv Exp Med Biol. 1995;378:75-8. doi: 10.1007/978-1-4615-1971-3_15.


Our previous studies demonstrated that monocytes, when cultured under certain conditions, are able to differentiate into DC-like cells (MoDC) presenting a high accessory activity and low phagocytic function. In the present study, we demonstrate that under the effect of a triple combination of IL-4, IFN-gamma and GM-CSF human blood monocytes are able to differentiate into the cells expressing an identical phenotype and functional features of blood dendritic cells. MoDC stimulated T cell proliferation 20-30 times higher than untreated monocytes, similar to blood DC. They expressed abundant HLA-DR molecules, but only trace amounts of the monocyte/macrophage markers CD16 (FcR III), CD32 (FcR II), and CD14. Phagocytosis of Ig- and complement-opsonized bacteria was reduced by 93%.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Differentiation / drug effects
  • Dendritic Cells / cytology*
  • Dendritic Cells / drug effects*
  • Dendritic Cells / immunology
  • Granulocyte-Macrophage Colony-Stimulating Factor / administration & dosage*
  • HLA-DR Antigens / metabolism
  • Humans
  • In Vitro Techniques
  • Interferon-gamma / administration & dosage*
  • Interleukin-4 / administration & dosage*
  • Lipopolysaccharide Receptors / metabolism
  • Lymphocyte Activation
  • Lymphocyte Culture Test, Mixed
  • Monocytes / cytology*
  • Monocytes / drug effects*
  • Monocytes / immunology
  • Phagocytosis / drug effects
  • Phenotype
  • Receptors, IgG / metabolism
  • T-Lymphocytes / immunology


  • HLA-DR Antigens
  • Lipopolysaccharide Receptors
  • Receptors, IgG
  • Interleukin-4
  • Interferon-gamma
  • Granulocyte-Macrophage Colony-Stimulating Factor