cDNA cloning and functional characterization of rat intestinal monocarboxylate transporter

Biochem Biophys Res Commun. 1995 Dec 5;217(1):370-7. doi: 10.1006/bbrc.1995.2786.

Abstract

A cDNA clone which encodes a monocarboxylate transporter (ratMCT1) was isolated from a rat small intestinal cDNA library, which was screened by using full-length MCT1 cDNA of Chinese hamster ovary cells. The ratMCT1 cDNA was sequenced and predicted a protein of 494 amino acids with twelve potential transmembrane domains. The amino acid sequence showed 93.1% and 84.6% identity to the hamster and human monocarboxylate transporters, respectively. When expressed in Xenopus laevis oocytes, the ratMCT1 cRNA caused a significant increase in the uptake of radiolabeled lactic acid. Poly(A)+ RNA transcripts hybridizing to the ratMCT1 cDNA were detected in rat brain, heart, kidney, lung, muscle and brain capillaries. These results indicate that MCT1 contributes to pH-dependent and carrier-mediated transport of monocarboxylic acids in many tissues, not just in the small intestine.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Brain / metabolism
  • CHO Cells
  • Carrier Proteins / genetics*
  • Carrier Proteins / metabolism
  • Cloning, Molecular
  • Cricetinae
  • DNA, Complementary / genetics*
  • Female
  • Humans
  • Intestine, Small / metabolism*
  • Molecular Sequence Data
  • Monocarboxylic Acid Transporters
  • Oocytes / metabolism
  • Rats
  • Rats, Wistar
  • Xenopus laevis

Substances

  • Carrier Proteins
  • DNA, Complementary
  • Monocarboxylic Acid Transporters

Associated data

  • GENBANK/D63834