Structural analysis of the O-antigen of the lipopolysaccharide of Rhizobium tropici CIAT899

Carbohydr Res. 1995 Oct 2;275(2):285-94. doi: 10.1016/0008-6215(95)00178-v.

Abstract

The structure of the O-antigen chain of the lipopolysaccharide isolated from Rhizobium tropici CIAT899, by the phenol-water procedure, and recovered from the phenol layer, has been investigated by hydrolysis, methylation analysis and 1D and 2D 1H and 13C NMR spectroscopy of the complete polysaccharide and of oligosaccharides obtained by partial hydrolysis. The O-antigen has the repeating unit [formula: see text]

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carbohydrate Sequence
  • Hydrolysis
  • Magnetic Resonance Spectroscopy
  • Methylation
  • Molecular Sequence Data
  • Monosaccharides / analysis
  • O Antigens / chemistry*
  • Oligosaccharides / chemistry
  • Rhizobium / immunology*

Substances

  • Monosaccharides
  • O Antigens
  • Oligosaccharides