Color Selection With a Hygromycin-Resistance-Based Escherichia Coli-Mycobacterial Shuttle Vector

Gene. 1995 Dec 1;166(1):181-2. doi: 10.1016/0378-1119(95)00597-x.

Abstract

Hygromycin-resistance (HyR)-based Escherichia coli-mycobacterial shuttle plasmids have high efficiencies of transformation and a broad mycobacterial host range. We have introduced a lacZ alpha (encoding the alpha-polypeptide fragment of beta-galactosidase (beta Gal))-multiple cloning site cassette into a HyR-based shuttle vector to generate a plasmid with nine unique cloning sites and the added feature of beta Gal color selection in appropriate E. coli host strains.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Cinnamates*
  • Drug Resistance, Microbial
  • Escherichia coli / genetics*
  • Gene Transfer Techniques
  • Genetic Vectors*
  • Hygromycin B / analogs & derivatives
  • Mycobacterium / genetics*
  • Restriction Mapping

Substances

  • Cinnamates
  • Hygromycin B
  • hygromycin A