Identification and Sequences of the Treponema Pallidum fliM', fliY, fliP, fliQ, fliR and flhB' Genes

Gene. 1995 Dec 1;166(1):57-64. doi: 10.1016/0378-1119(95)00583-x.

Abstract

Information regarding the biology and virulence attributes of Treponema pallidum (Tp) is limited due to the lack of genetic exchange mechanisms and the inability to continuously cultivate this spirochete. We have utilized TnphoA mutagenesis of a Tp genomic DNA library in Escherichia coli (Ec) to identify genes encoding exported proteins, a subset of which are likely to be important in treponemal pathogenesis. We report here the identification and nucleotide (nt) sequence of a 5-kb treponemal DNA insert that contains seven open reading frames (ORFs). The proteins encoded by six of these ORFs have homology with members of a newly described protein family involved in the biogenesis/assembly of flagella and the control of flagellar rotation in Ec, Salmonella typhimurium (St) and Bacillus subtilis (Bs). Certain members of this family are also involved in the export of virulence factors in Yersinia (Yr) spp., St and Shigella flexneri (Sf). We have named these six ORFs fliM', fliY, fliP, fliQ, fliR and flhB'. The operon containing these ORFs has been designated as the fla operon. We hypothesize that the protein products of these genes are involved in the biogenesis/assembly of flagella and the control of flagellar rotation in Tp.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Bacterial Proteins / chemistry
  • Bacterial Proteins / genetics*
  • Base Sequence
  • DNA Transposable Elements
  • DNA, Bacterial / genetics
  • Escherichia coli Proteins*
  • Flagella / ultrastructure*
  • Genes, Bacterial*
  • Membrane Proteins*
  • Molecular Sequence Data
  • Morphogenesis
  • Mutagenesis, Insertional
  • Open Reading Frames
  • Operon
  • Restriction Mapping
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Solubility
  • Treponema pallidum / genetics*
  • Treponema pallidum / pathogenicity

Substances

  • Bacterial Proteins
  • DNA Transposable Elements
  • DNA, Bacterial
  • Escherichia coli Proteins
  • FlhB protein, Bacteria
  • FliQ protein, E coli
  • FliR protein, Bacteria
  • Membrane Proteins
  • FliM protein, Bacteria
  • FliP protein, Bacillus
  • FliQ protein, Bacteria
  • FliY protein, Bacteria

Associated data

  • GENBANK/U36839