Functional association between the human myeloid immunoglobulin A Fc receptor (CD89) and FcR gamma chain. Molecular basis for CD89/FcR gamma chain association

J Biol Chem. 1995 Dec 15;270(50):29781-7. doi: 10.1074/jbc.270.50.29781.

Abstract

FcR gamma chain has previously been shown to interact with the TCR-CD3 complex, the IgE Fc receptor I (Fc epsilon RI), and the class I and IIIA IgG receptors (Fc gamma RI and Fc gamma RIIIa). Here, we demonstrate that the Fc receptor gamma chain associates with Fc alpha R in transfected IIA1.6 B lymphocytes. Fc alpha R could be expressed at the surface of IIA1.6 B cells by itself, but was devoid of signaling capacity. Upon co-expression of FcR gamma chain, a physical interaction with Fc alpha R could be demonstrated. This association proved crucial for the triggering of both proximal (intracellular calcium increase and tyrosine phosphorylation), as well as distal (IL-2 release), signal transduction responses. We next tested the hypothesis that a positively charged arginine residue (Arg209) within the transmembrane domain of Fc alpha R promotes association with FcR gamma chain. We therefore constructed Fc alpha R molecules where Arg209 was mutated to either a positively charged histidine, a negatively charged aspartic acid, or an uncharged leucine. A functional association between Fc alpha R and FcR gamma chain was observed only with a positively charged residue (Arg209 or His209) present within the Fc alpha R transmembrane domain. These data show that transmembrane signal transduction by the Fc alpha R is mediated via FcR gamma chain, and that Fc alpha R requires a positively charged residue within the transmembrane domain to promote functional association.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antigens, CD / biosynthesis
  • Antigens, CD / isolation & purification
  • Antigens, CD / metabolism*
  • Arginine
  • B-Lymphocytes
  • Base Sequence
  • Cell Line
  • Cell Membrane / immunology
  • DNA Primers
  • Gene Expression
  • Histidine
  • Humans
  • Immunoglobulin A / metabolism
  • Interleukin-2 / biosynthesis
  • Kinetics
  • Leukemia, Myeloid
  • Mice
  • Molecular Sequence Data
  • Mutagenesis, Site-Directed
  • Point Mutation
  • Polymerase Chain Reaction
  • Receptors, Fc / biosynthesis
  • Receptors, Fc / isolation & purification
  • Receptors, Fc / metabolism*
  • Receptors, IgG / biosynthesis
  • Receptors, IgG / isolation & purification
  • Receptors, IgG / metabolism*
  • Recombinant Proteins / biosynthesis
  • Recombinant Proteins / isolation & purification
  • Recombinant Proteins / metabolism
  • Transfection
  • Tumor Cells, Cultured

Substances

  • Antigens, CD
  • DNA Primers
  • Fc(alpha) receptor
  • Immunoglobulin A
  • Interleukin-2
  • Receptors, Fc
  • Receptors, IgG
  • Recombinant Proteins
  • Histidine
  • Arginine