Analyzing protein-protein interactions using two-hybrid system

Methods Enzymol. 1995;254:241-63. doi: 10.1016/0076-6879(95)54018-0.
No abstract available

MeSH terms

  • Animals
  • Base Sequence
  • Cell Line
  • Cloning, Molecular / methods
  • DNA Primers
  • DNA-Binding Proteins / metabolism*
  • Escherichia coli / genetics
  • Gene Library
  • Genetic Markers
  • Genetic Vectors
  • Genotype
  • Humans
  • Indicators and Reagents
  • Molecular Sequence Data
  • Plasmids*
  • Polymerase Chain Reaction / methods
  • Promoter Regions, Genetic
  • Protein Multimerization*
  • Proteins / chemistry
  • Proteins / metabolism*
  • Recombinant Proteins / biosynthesis*
  • Recombinant Proteins / metabolism
  • Restriction Mapping
  • Saccharomyces cerevisiae / genetics
  • Transcription Factors / metabolism*
  • Transfection / methods
  • Two-Hybrid System Techniques

Substances

  • DNA Primers
  • DNA-Binding Proteins
  • Genetic Markers
  • Indicators and Reagents
  • Proteins
  • Recombinant Proteins
  • Transcription Factors