A subclass of bHLH proteins required for cardiac morphogenesis

Science. 1995 Dec 22;270(5244):1995-9. doi: 10.1126/science.270.5244.1995.

Abstract

Skeletal muscle development is controlled by a family of muscle-specific basic helix-loop-helix (bHLH) transcription factors. Two bHLH genes, dHAND and eHAND, have now been isolated that are expressed in the bilateral heart primordia and subsequently throughout the primitive tubular heart and its derivatives during chick and mouse embryogenesis. Incubation of stage 8 chick embryos with dHAND and eHAND antisense oligonucleotides revealed that either oligonucleotide alone had no effect on embryonic development, whereas together they arrested development at the looping heart tube stage. Thus, dHAND and eHAND may play redundant roles in the regulation of the morphogenetic events of vertebrate heart development.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Actins / genetics
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Basic Helix-Loop-Helix Transcription Factors
  • Cardiovascular System / embryology
  • Cardiovascular System / metabolism
  • Chick Embryo
  • DNA-Binding Proteins / biosynthesis
  • DNA-Binding Proteins / chemistry
  • DNA-Binding Proteins / genetics*
  • DNA-Binding Proteins / physiology
  • Embryonic and Fetal Development
  • Gene Expression
  • Heart / embryology*
  • Helix-Loop-Helix Motifs*
  • In Situ Hybridization
  • MEF2 Transcription Factors
  • Mesoderm / metabolism
  • Mice
  • Molecular Sequence Data
  • Morphogenesis
  • Myocardium / metabolism
  • Myogenic Regulatory Factors*
  • Oligonucleotides, Antisense / pharmacology
  • Transcription Factors / biosynthesis
  • Transcription Factors / chemistry
  • Transcription Factors / genetics*
  • Transcription Factors / physiology
  • Zebrafish Proteins

Substances

  • Actins
  • Basic Helix-Loop-Helix Transcription Factors
  • DNA-Binding Proteins
  • Hand1 protein, mouse
  • Hand2 protein, mouse
  • MEF2 Transcription Factors
  • Mef2c protein, mouse
  • Myogenic Regulatory Factors
  • Oligonucleotides, Antisense
  • Transcription Factors
  • Zebrafish Proteins
  • hand2 protein, zebrafish
  • helix-loop-helix protein, eHAND

Associated data

  • GENBANK/U40039
  • GENBANK/U40040
  • GENBANK/U40041