[Study on conservative treatment of retinoblastoma--effect of intravitreal injection of melphalan on the rabbit retina]

Nippon Ganka Gakkai Zasshi. 1995 Nov;99(11):1230-5.
[Article in Japanese]


Effects of an intravitreal injection of melphalan on the electroretinogram and on the retinal structure were studied in albino rabbits to establish the non-toxic dose for its intravitreal use. The a-wave, the b-wave, the c-wave, the oscillatory potential and the retinal structure remained unchanged after 10-micrograms injection, but moderately changed after 20-micrograms injection and greatly deteriorated after 90-micrograms injection. A 10-micrograms injection is equal to an intravitreal concentration of about 5.9 micrograms/ml, if evenly diffused in the rabbit vitreous. Considering that colony formation of in vitro retinoblastoma cells is completely suppressed by melphalan at 4 micrograms/ml concentration, an intravitreal application of melphalan could be used as a non-surgical treatment for retinoblastoma.

Publication types

  • English Abstract

MeSH terms

  • Animals
  • Antineoplastic Agents, Alkylating / administration & dosage*
  • Antineoplastic Agents, Alkylating / pharmacology
  • Electroretinography / drug effects
  • Eye Neoplasms / drug therapy*
  • Injections
  • Melphalan / administration & dosage*
  • Melphalan / pharmacology
  • Rabbits
  • Retina / drug effects*
  • Retinoblastoma / drug therapy*
  • Vitreous Body


  • Antineoplastic Agents, Alkylating
  • Melphalan