Putative Leishmania hybrids in the Eastern Andean valley of Huanuco, Peru

Acta Trop. 1995 Aug;59(4):293-307. doi: 10.1016/0001-706x(95)00094-u.


During an outbreak of tegumentary leishmaniasis that developed in the 1990s in the Eastern Andean valley of Huanuco, Peru, the coexistence of Andean (uta) and sylvatic leishmaniases was suspected for ecological and geographical reasons, and sympatric sampling was carried out. Seven human isolates of Leishmania were characterized by multilocus enzyme electrophoresis, random amplification of polymorphic DNA and molecular karyotyping. The three methods identified 3 isolates as L. braziliensis, and 4 isolates as putative hybrids with characters of L. braziliensis and L. peruviana. Data from Huanuco are compared to previous results from other areas endemic for uta. Biological and epidemiological implications are discussed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Humans
  • Isoenzymes / analysis
  • Karyotyping
  • Leishmania / classification*
  • Leishmania / enzymology
  • Leishmania / genetics
  • Leishmania braziliensis / classification*
  • Leishmania braziliensis / enzymology
  • Leishmania braziliensis / genetics
  • Leishmaniasis, Cutaneous / parasitology*
  • Molecular Sequence Data
  • Peru
  • Phylogeny
  • Protozoan Proteins / analysis
  • Random Amplified Polymorphic DNA Technique


  • Isoenzymes
  • Protozoan Proteins