Cloning, sequencing, and expression in Escherichia coli of the Bacillus pumilus gene for ferulic acid decarboxylase

Appl Environ Microbiol. 1995 Dec;61(12):4484-6. doi: 10.1128/AEM.61.12.4484-4486.1995.

Abstract

The Bacillus pumilus gene encoding a ferulic acid decarboxylase (fdc) was identified and isolated by its ability to promote ferulic acid decarboxylation in Escherichia coli DH5 alpha. The DNA sequence of the fdc gene was determined, and the recombinant enzyme produced in E. coli was purified and characterized.

MeSH terms

  • Amino Acid Sequence
  • Bacillus / enzymology*
  • Bacillus / genetics
  • Base Sequence
  • Carboxy-Lyases / genetics*
  • Cloning, Molecular
  • Escherichia coli / genetics
  • Molecular Sequence Data
  • Restriction Mapping

Substances

  • Carboxy-Lyases
  • phenylacrylic acid decarboxylase

Associated data

  • GENBANK/X84815