A self-defense gene homologous to tetracycline effluxing gene essential for antibiotic production in Streptomyces aureofaciens

Biosci Biotechnol Biochem. 1995 Oct;59(10):1835-41. doi: 10.1271/bbb.59.1835.


By Northern blot analyses with DNA probes carrying 6-demethylchlortetracycline (6-DCT) biosynthetic genes from Streptomyces aureofaciens NRRL3203, a highly expressed gene (tcrC) was detected in a high titer producing mutant derived from the parental strain NRRL3203 by NTG mutagenesis. The analysis of the nucleotide sequence of the 2.8-kb BamHI fragment containing tcrC gene showed that the predicted tcrC gene product is a protein consisting of 512 amino acids. The deduced amino acid sequence had a high level identity with that of the self-defense gene (tet347) of Streptomyces rimosus, known to mediate oxytetracycline efflux. The tcrC gene-inactivated strains generated from strain NRRL3203 by gene replacement had a 90% decrease in the level of resistance to tetracycline and the antibiotic productivity when compared with the parental strain.

MeSH terms

  • Amino Acid Sequence
  • Anti-Bacterial Agents* / metabolism
  • Anti-Bacterial Agents* / pharmacology
  • Base Sequence
  • Cloning, Molecular
  • DNA, Bacterial
  • Gene Expression
  • Genes, Bacterial*
  • Molecular Sequence Data
  • Sequence Homology, Amino Acid
  • Streptomyces aureofaciens / genetics*
  • Streptomyces aureofaciens / metabolism
  • Tetracycline* / metabolism
  • Tetracycline* / pharmacology


  • Anti-Bacterial Agents
  • DNA, Bacterial
  • Tetracycline

Associated data

  • GENBANK/D38215