Stabilization of the tight junction of the intestinal Caco-2 cell monolayer by milk whey proteins

K Hashimoto; K Takeda; T Nakayama; M Shimizu

doi:10.1271/bbb.59.1951

Stabilization of the tight junction of the intestinal Caco-2 cell monolayer by milk whey proteins

Biosci Biotechnol Biochem. 1995 Oct;59(10):1951-2. doi: 10.1271/bbb.59.1951.

Authors

K Hashimoto¹, K Takeda, T Nakayama, M Shimizu

Affiliation

¹ School of Food and Nutritional Sciences, University of Shizuoka, Japan.

PMID: 8534990
DOI: 10.1271/bbb.59.1951

Abstract

The tight junction (TJ) of the human intestinal Caco-2-SF monolayer was shown not to have been stably formed. TJ was stabilized by incubating the cell monolayer with beta-lactoglobulin (beta-Lg) and bovine serum albumin (BSA). Thus, the Caco-2-SF cell-culture system will provide a useful model for studying the factors which stabilize TJ and for investigating the mechanism of TJ regulation.

MeSH terms

Animals
Caco-2 Cells
Cattle
Cell Membrane Permeability / drug effects
Humans
Intestinal Mucosa / cytology
Lactoglobulins / metabolism
Lactoglobulins / pharmacology
Milk
Milk Proteins / pharmacology*
Serum Albumin, Bovine / metabolism
Serum Albumin, Bovine / pharmacology
Tight Junctions / drug effects*
Tight Junctions / metabolism
Trypsin / metabolism
Whey Proteins

Substances

Lactoglobulins
Milk Proteins
Whey Proteins
Serum Albumin, Bovine
Trypsin