Cloning and sequencing of V genes from anti-osteosarcoma monoclonal antibodies TP-1 and TP-3: location of lysine residues and implications for radiolabeling

Nucl Med Biol. 1995 Aug;22(6):765-71. doi: 10.1016/0969-8051(95)00014-o.


Monoclonal antibodies TP-1 and TP-3 are of potential utility for the radioimmunodiagnosis of osteosarcoma in both human and canine patients. The V genes of these antibodies were cloned and sequenced and to facilitate radiolabeling of these proteins, the location of the lysine residues within these sequences have been determined. The V-domains of TP-1 contain a total of 12 lysines, 10 in the framework region and 2 in the CDR region, while the V-domains of TP-3 contain a total of 14 lysines, 11 in the framework region and 3 in the CDR regions. Using space-filling models, the availability of each lysine residue for radiolabeling, and potential interference with antigen binding was predicted.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antibodies, Monoclonal / biosynthesis*
  • Antibodies, Monoclonal / chemistry
  • Base Sequence
  • Cloning, Molecular / methods
  • Crystallography, X-Ray
  • DNA Primers
  • Dog Diseases
  • Dogs
  • Genes, Immunoglobulin*
  • Humans
  • Immunoglobulin Heavy Chains / biosynthesis
  • Immunoglobulin Heavy Chains / chemistry
  • Immunoglobulin Light Chains / biosynthesis
  • Immunoglobulin Light Chains / chemistry
  • Immunoglobulin Variable Region / biosynthesis*
  • Immunoglobulin Variable Region / chemistry
  • Lysine*
  • Models, Molecular
  • Molecular Sequence Data
  • Osteosarcoma / diagnostic imaging*
  • Osteosarcoma / immunology*
  • Osteosarcoma / veterinary
  • Polymerase Chain Reaction
  • Protein Conformation*
  • Radioimmunodetection*


  • Antibodies, Monoclonal
  • DNA Primers
  • Immunoglobulin Heavy Chains
  • Immunoglobulin Light Chains
  • Immunoglobulin Variable Region
  • Lysine