Common clonal chromosome aberrations in cytokine-dependent continuous human T-lymphocyte cell lines

Cancer Genet Cytogenet. 1995 Nov;85(1):68-71. doi: 10.1016/0165-4608(95)00118-2.


Antigen-mediated T-cell proliferation is a transient phenomenon. Like other somatic cells, T lymphocytes generally show replicative senescence in vitro. However, we here show that cytokine-dependent continuous (immortal) T-cell lines can be established from skin biopsy specimens of inflammatory skin diseases. Continuous growth can be obtained by culturing T cells in medium supplemented with interleukin-2 and interleukin-4, but without antigen or antigen-presenting cells added. Loss of the T-cell antigen receptor complex is observed in some of the continuous T-cell lines. Most T-cell lines develop clonal chromosome aberrations during continuous growth. Aberrations for chromosomes 1, 2, 8, 16, and 18 are most commonly observed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Division
  • Cell Line
  • Chromosome Aberrations*
  • Cytokines / pharmacology*
  • Dermatitis / pathology
  • Humans
  • Immunophenotyping
  • Interleukin-2 / pharmacology
  • Interleukin-4 / pharmacology
  • Lymphoma, T-Cell, Cutaneous / genetics*
  • Lymphoma, T-Cell, Cutaneous / pathology
  • Skin
  • Skin Neoplasms / genetics*
  • Skin Neoplasms / pathology
  • T-Lymphocytes / immunology
  • T-Lymphocytes / ultrastructure*
  • Tumor Cells, Cultured


  • Cytokines
  • Interleukin-2
  • Interleukin-4