Remarks on the vitality of the human cornea after organ culture

Cornea. 1995 Sep;14(5):502-8.


The purpose of this study was to obtain further information on the viability of organ-cultured human cornea. We thus used a specific staining method for succinate dehydrogenase (SDH), which is located in the membrane system of vital mitochondria. We examined fresh and long-term-cultured human corneas. After an initial incubation period in dextran-free culture medium, corneas were stored in a medium containing dextran. With respect to different appearances of the SDH staining, minimal essential medium without dextran seems to have a positive effect on the condition of epithelial cells. After renewal of the medium, keratocytes showed a brief improvement followed by a delayed deterioration, while the endothelial cells were severely damaged. However, best results for all three cell types were observed on the fourth day in a medium containing dextran. We therefore conclude that these corneas were best suited for transplantation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Aging / physiology
  • Cell Survival / physiology
  • Child
  • Child, Preschool
  • Cornea / cytology
  • Cornea / enzymology
  • Cornea / physiology*
  • Corneal Stroma / cytology
  • Corneal Stroma / physiology
  • Culture Media
  • Endothelium, Corneal / cytology
  • Endothelium, Corneal / physiology
  • Humans
  • Infant
  • Infant, Newborn
  • Middle Aged
  • Mitochondria / enzymology
  • Organ Culture Techniques*
  • Organ Preservation
  • Succinate Dehydrogenase / metabolism


  • Culture Media
  • Succinate Dehydrogenase