Isolation of a cDNA encoding a UV-damaged DNA binding factor defective in xeroderma pigmentosum group E cells

Mutat Res. 1996 Jan 2;362(1):105-17. doi: 10.1016/0921-8777(95)00040-2.

Abstract

XPE binding factor (XPE-BF) is deficient in a subset of patients from xeroderma pigmentosum complementation group E. Binding activity copurifies with a 125 kDa polypeptide (p125) that binds to DNA damaged by ultraviolet (UV) radiation and many other agents. We isolated cDNA encoding a polypeptide with a predicted amino acid sequence that matched the sequences of eleven tryptic peptides derived from digestion of XPE-BF purified from human placenta. In vitro transcription and translation of the cDNA yielded a polypeptide of 125 kDa that bound specifically to UV-damaged DNA. Therefore the cDNA encodes either all or the major component of XPE-BF.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Binding Sites
  • Cloning, Molecular
  • Conserved Sequence
  • DNA / radiation effects
  • DNA Damage*
  • DNA Repair / physiology*
  • DNA, Complementary / isolation & purification
  • DNA-Binding Proteins / chemistry
  • DNA-Binding Proteins / genetics*
  • Haplorhini
  • Humans
  • Molecular Sequence Data
  • Peptide Fragments / chemistry
  • Protein Biosynthesis
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Transcription, Genetic
  • Ultraviolet Rays

Substances

  • DDB1 protein, human
  • DNA, Complementary
  • DNA-Binding Proteins
  • Peptide Fragments
  • DNA

Associated data

  • GENBANK/U32986