Requirement for Xist in X chromosome inactivation

Nature. 1996 Jan 11;379(6561):131-7. doi: 10.1038/379131a0.


The Xist gene has been proposed as a candidate for the X inactivation centre, the master regulatory switch locus that controls X chromosome inactivation. So far this hypothesis has been supported solely by indirect evidence. Here we describe gene targeting of Xist, and provide evidence for its absolute requirement in the process of X chromosome inactivation.

MeSH terms

  • Alleles
  • Animals
  • Base Sequence
  • Cell Line
  • Chimera
  • Clone Cells
  • DNA Primers
  • Dosage Compensation, Genetic*
  • Embryo, Mammalian
  • Female
  • Gene Targeting
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C3H
  • Molecular Sequence Data
  • Mutagenesis
  • RNA, Long Noncoding
  • RNA, Untranslated*
  • Restriction Mapping
  • Stem Cells
  • Transcription Factors / genetics*
  • X Chromosome / genetics


  • DNA Primers
  • RNA, Long Noncoding
  • RNA, Untranslated
  • Transcription Factors
  • XIST non-coding RNA