A rapid (4--6-hour) urine-culture system for direct identification and direct antimicrobial susceptibility testing

Am J Clin Pathol. 1979 Feb;71(2):177-83. doi: 10.1093/ajcp/71.2.177.

Abstract

This study evaluates a new direct rapid system for urine cultures, including detection and quantitation of positive specimens by Gram stain, direct identification by 4--6-hour incubation of sediment with reagent strips, and antibiotic susceptibility testing by direct (3--4-hour) disk-elution methods. Of 987 routine urine specimens, 121 had significant (less than or equal to 10(5) colony-forming units/ml) gram-negative bacilluria, of which 89% were detected by the Gram stain. Direct rapid identification was correct in 94%. Results of direct disk-elution antimicrobial tests showed overall agreement with results of standard disk diffusion of 93% of tests, and major discrepancies in 4%. For urine specimens with gram-negative bacilluria, this system permitted detection, quantitation, identification, and antimicrobial susceptibility testing in four to six hours with reasonable, though not complete, accuracy.

MeSH terms

  • Anti-Bacterial Agents / pharmacology
  • Bacteria / classification
  • Bacteria / drug effects
  • Bacteria / isolation & purification
  • Bacteriuria / microbiology
  • Drug Resistance, Microbial
  • Female
  • Humans
  • Immunodiffusion / methods
  • Male
  • Microbial Sensitivity Tests*
  • Staining and Labeling / methods
  • Time Factors
  • Urinary Tract Infections / microbiology*
  • Urine / microbiology*

Substances

  • Anti-Bacterial Agents