Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
, 276 (2), 365-86

Structure of the O-deacetylated Glucuronoxylomannan From Cryptococcus Neoformans Serotype C as Determined by 2D 1H NMR Spectroscopy

Affiliations

Structure of the O-deacetylated Glucuronoxylomannan From Cryptococcus Neoformans Serotype C as Determined by 2D 1H NMR Spectroscopy

B E Bacon et al. Carbohydr Res.

Abstract

The primary structure of the O-deacetylated capsular glucuronoxylomannan (GXM) isolated from Cryptococcus neoformans serotype C was investigated by 2D NMR spectroscopy. Assignment of the 1H NMR chemical shifts for the polysaccharide was accomplished from the analysis of DQF-COSY, TOCSY, NOESY and/or ROESY spectra of three isolates (298, 34, and 401). These isolates contain the same polysaccharide glycosyl residues but in different proportions. The serotype C GXM consists of two repeating polysaccharide units that have the following structures: [formula: see text] It is not known if these repeating units comprise a single or two separate polymer chains. The relative amounts of the more highly branched octasaccharide 1 in the isolates studied (i.e., approximately 75% in isolate 34, 50% in isolate 298, and 25% in isolate 401) can be used to explain the serological specificity of these isolates with C. neoformans factor sera, as was previously determined by ELISA in this laboratory. The octasaccharide 1 component is the one previously postulated as the structure of the serotype C GXM although definitive placement of the beta-Xyl-(1-->4) residues had previously not been determined. The heptasaccharide 2 component is uniformly found as the repeating unit in the polysaccharide from serotype B isolates. Additionally, GXM 401 was found to contain a small amount of the hexasaccharide repeating unit usually attributed to serotype A GXM.

Similar articles

See all similar articles

Cited by 5 PubMed Central articles

Publication types

LinkOut - more resources

Feedback